Many regulators of angiogenesis have been identified, validated, and developed based on their effects on EC proliferation. In this technique, glucose, oligomycin inhibitor of ATP synthase in the electron transport chain ETCand 2-deoxy-glucose 2-DG, inhibitor of hexokinase are sequentially administered while measurements of ECAR are being performed.
Gene delivery via AAV represents a reliable and relatively inexpensive approach for gene therapy in inherited or acquired vascular disease models.
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HGPs constitute a good candidate biomarker. It is often not possible to accurately visualize the process of angiogenesis and its molecular players. Many of these conditions share characteristics, for example the occurrence of hypoxia or inflammation, recruitment of inflammatory cells, angiogenic growth factor production, basement membrane degradation, endothelial cell EC migration, proliferation and differentiation, and modulation of vascular support cells.
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